标签：Megazyme官网

For the specific and rapid assay of L-arginine, urea and ammonia in grape juice/must and wine. Content:150 assays – 50 of each component
UV-method for the determination of L-Arginine, Urea and Ammonia 
in grape juice, must and wine
Principle:
                            (microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH₄⁺ → L-glutamic acid + NADP⁺ + 
H₂O
                     (urease)
(2) Urea + H₂O → 2 NH₃ + CO₂
(arginase)
(3) L-Arginine + H₂O → urea + ornithine

Kit size:                             50 assays
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 20 min [ammonia (2 min), urea (6 min),
L-arginine (7 min)] Detection limit:                  0.07 mg/L (ammonia)
0.13 mg/L (urea)
0.37 mg/L (L-arginine)
Application examples:
Grape juice, wine must, wine and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:          Improved method
Advantages

• Improved assay format
• Very rapid reactions due to use of uninhibited glutamate dehydrogenase
• All enzymes supplied as stabilised suspensions
• Very competitive price (cost per test)
• All reagents stable for > 2 years after preparation
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included
• Extended cofactors stability

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