For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit
Colourimetric method for the determination of Yeast and
Mushroom β-Glucan in yeast, mushroom, foodstuffs and
other materials
Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O →
soluble glucan
(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)
(
exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose
(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
(amyloglucosidase)
(6) α-Glucan + H2O → D-glucose
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 100 min
Detection limit: 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition: Novel method
Advantages
- Very cost effective
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
