适用样品：啤酒、果汁、软饮料、牛奶、果酱、蜂蜜、面包、焙烤产品、乳制品、糖果、甜点、糖食、冰淇淋、水果和蔬菜（如：马铃薯）、肉制品（如香肠）、调味品、饲料、烟、化妆品、纸及其他原料（如生物培养、样品等）；
The Sucrose/Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4
oC.
UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials
Principle:
(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose
(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP
(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP
(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+
(phosphoglucose isomerase)
(5) F-6-P → G-6-P
Kit size: 100 assays of each
Method: Spectrophotometric at 340 nm
Reaction time: ~ 30 min
Detection limit: 1.38 mg/L
Application examples:
Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread,
bakery products, dairy products, candies, desserts, confectionery, sweets,
ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage),
condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics,
pharmaceuticals, paper and other materials
Method recognition:
Methods based on this principle have been accepted by NF, EN, NEN,
DIN, GOST, IFU, AIJN, MEBAK and IOCCC
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Stabilised D-glucose / D-fructose standard solution included
- Extended cofactors stability
